How do Revivicor scientists edit pig egg DNA in the lab?

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Revivicor Scientists Genetically Modify Pig Eggs for Transplantation Through a Precise Laboratory Procedure

Revivicor scientists genetically modify pig eggs by first removing the original DNA from a pig oocyte using a pipette. Subsequently, these enucleated eggs are injected with cells that contain the necessary genetic modifications and all the instructions required for development. These edited eggs are then implanted into surrogate sows, with the genetically modified piglets born approximately four months later. This intricate process is central to Revivicor's efforts in xenotransplantation, aiming to address the critical shortage of human organs.
DNA Removal and Genetic Injection

In the Revivicor Laboratories in Blacksburg, Virginia, a detailed procedure is undertaken to alter pig oocytes. Todd Vaught, head of cell biology and nuclear transfer at Revivicor, demonstrated how a pipette is used to extract the DNA from a pig egg. This step is crucial for preparing the egg to receive new genetic material. Following the removal of the native DNA, the oocyte is injected with specialized cells. These cells are engineered to carry specific genetic modifications and contain all the essential instructions needed to guide the development of a genetically modified pig.
Implantation and Gestation

Once the pig eggs have been successfully edited in the laboratory, they are prepared for implantation. These genetically modified eggs are then transferred into surrogate mother pigs, known as sows. The process of gestation in pigs typically lasts around four months. After this period, new litters of genetically modified piglets are born. These offspring are the result of the precise genetic engineering performed on the oocytes, carrying the intended modifications designed to make their organs more compatible with human recipients.
The Goal of Xenotransplantation

This sophisticated laboratory work is fundamental to Revivicor's broader mission of advancing xenotransplantation. The company is focused on developing genetically modified pigs whose organs, such as kidneys and hearts, can be transplanted into human patients. This research is particularly significant given the chronic shortage of human organs available for transplantation, which leads to thousands of deaths annually in the United States alone. By genetically modifying the pigs, Revivicor aims to reduce the likelihood of immune rejection, a major hurdle in transplanting animal organs into humans.

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How do Revivicor scientists edit pig egg DNA in the lab?

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